Fig. 8: LTP is prevented in 2- to 3-month-old female Arg1-cKO mice. | Nature Neuroscience

Fig. 8: LTP is prevented in 2- to 3-month-old female Arg1-cKO mice.

From: ARG1-expressing microglia show a distinct molecular signature and modulate postnatal development and function of the mouse brain

Fig. 8: LTP is prevented in 2- to 3-month-old female Arg1-cKO mice.The alternative text for this image may have been generated using AI.

a, Time course of fEPSPs before and after LTP induction in male Arg1-control (blue circles; n = 8), female Arg1-control (red circles; n = 8), male Arg1-cKO (light blue circles; n = 9) and female Arg1-cKO (light red circles; n = 13) mice. The inset traces show fEPSPs before (1) and 60 min (2) and 120 min (3) after the plasticity protocol. Arg1-control male: 161 ± 7% (E-LTP) and 167 ± 7 % (L-LTP); Arg1-control female: 157 ± 6% (E-LTP) and 165 ± 6% (L-LTP); Arg1-cKO male: 129 ± 6% (E-LTP) and 139 ± 6% (L-LTP); Arg1-cKO female: 114 ± 3% (E-LTP) and 112 ± 4% (L-LTP). b,c, Histograms show a summary of the results for E-LTP (b) and L-LTP (c). E-LTP, Arg1-control male versus Arg1-cKO male, P = 0.003; E-LTP, Arg1-control female versus Arg1-cKO female, P < 0.001; E-LTP, Arg1-cKO male versus Arg1-cKO female, P = 0.015; L-LTP, Arg1-control male versus Arg1-cKO male, P = 0.006; L-LTP, Arg1-control female versus Arg1-cKO female, P < 0.001; L-LTP, Arg1-cKO male versus Arg1-cKO female, P < 0.001. d, Short-term synaptic potentiation (STP) is not affected in any of the groups studied. Arg1-control male: 196 ± 17%, n = 8; Arg1-control female: 178 ± 17%, n = 8; Arg1-cKO male: 171 ± 16%, n = 9; Arg1-cKO female: 161 ± 14%, n = 13. e, PPR summary data. Arg1-control male: 1.53 ± 0.1 after LTP versus 1.52 ± 0.08 at baseline, n = 7; Arg1-control female: 1.84 ± 0.15 after LTP versus 1.75 ± 0.12 at baseline, n = 8; Arg1-cKO male: 1.69 ± 0.14 after LTP versus 1.61 ± 0.13 at baseline, n = 9; Arg1-cKO female: 1.49 ± 0.08 after LTP versus 1.78 ± 0.16 at baseline, n = 10; PPF, paired-pulse facilitation. f, Input–output (I/O) curves for Arg1-control male (n = 6), Arg1-control female (n = 6), Arg1-cKO male (n = 6) and Arg1-cKO female (n = 6) mice. The number of slices is shown in parentheses. Data are shown as mean ± s.e.m. Statistically significant differences were determined by unpaired two-sided t-tests, with P values corrected using the Bonferroni method for multiple comparisons; *P < 0.05 and **P < 0.01.

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