Extended Data Fig. 9: m6A hypomethylation of the endogenous C9 repeat-containing intron and YTHDC1 expression in the postmortem brain tissues. | Nature Neuroscience

Extended Data Fig. 9: m6A hypomethylation of the endogenous C9 repeat-containing intron and YTHDC1 expression in the postmortem brain tissues.

From: Globally reduced N6-methyladenosine (m6A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration

Extended Data Fig. 9: m6A hypomethylation of the endogenous C9 repeat-containing intron and YTHDC1 expression in the postmortem brain tissues.The alternative text for this image may have been generated using AI.

a, MeRIP coupled with RT-qPCR of endogenous C9 repeat-containing intronic RNA in control and C9ORF72-ALS/FTD iPSNs. Points represent individual cell lines. n = 6 per group. *P = 0.0427. b, Representative IHC staining of YTHDC1 at temporal cortex section of control and C9ORF72-ALS/FTD samples. Scale bar = 50 μm. c, Western blotting (left) and quantification (right) of YTHDC1 using postmortem motor cortex tissue samples. n = 3 individuals per group. *Non-specific bands. *P = 0.0246. (a, c) Data are mean ± s.e.m. Points represent individual control or patient samples. P values were calculated by two-tailed t test.

Source data

Back to article page