Extended Data Fig. 1: Endogenous ISL1 and LHX3 expression decreases with time.

a, Endogenous Il1 and Lhx3 expression decrease sharply after their peak at E13.5 (RNAseq data from2; n = 2-3 mice per timepoint; expression for each time point normalized to values at E13.5). b, Endogenous ISL1 and LHX3 are undetectable by immunofluorescence in CHAT+ cells in the L4-L5 ventral horn in untreated animals at P45. Images are maximum intensity projections of 15 z sections taken 3 µm apart. Scale bar represents 20 µM. Similar immunostaining experiments were performed in n = 12 mice.