Figure 11 | Scientific Reports

Figure 11

From: The Herpesvirus Nuclear Egress Complex Component, UL31, Can Be Recruited to Sites of DNA Damage Through Poly-ADP Ribose Binding

Figure 11The alternative text for this image may have been generated using AI.

UL31 binds directly to PAR. (A) Western blot of GFP-TRAP purified EGFP and EGFP-UL31 probed with a GFP monoclonal antibody. Migration of molecular weight markers (in kDa) are shown on the left of the blot. The approximately 60 kDa species found in the EGFP lane likely represents an EGFP dimer that was not denatured prior to, or during, electrophoresis. The blot has been cropped above 80 kDa and below 25 kDa. The full length blot is provided as supplementary information. (B) Purified BSA, EGFP and EGFP-UL31 were spotted onto PVDF membranes in duplicate. One sample was probed for EGFP using a GFP monoclonal antibody and the other was first incubated in PAR polymer and subsequently probed for PAR using anti-PAR antibodies. The results from two independent experiments are shown.

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