Figure 12 | Scientific Reports

Figure 12

From: The Herpesvirus Nuclear Egress Complex Component, UL31, Can Be Recruited to Sites of DNA Damage Through Poly-ADP Ribose Binding

Figure 12The alternative text for this image may have been generated using AI.

UL31 is preferentially recruited to sites of laser microirradiation in HSV-2 infected cells. (A) HeLa/EGFP31 cells were either mock-infected (uninfected) or infected with HSV-2 at an MOI of 3 for 5.5 h prior to microirradiation and time lapse analysis. Representative images from time lapse experiments before irradiation (Pre), immediately after irradiation (Post) and 30 s post irradiation are shown. Arrowheads indicate sites of laser microirradiation. Arrow indicates concentration of EGFP-UL31 at the nuclear rim, a surrogate indicator of UL34 expression and virus infection. (B) Quantitative analysis of UL31 recruitment to sites of laser microirradiation. n = 19 for infected cells in two independent experiments, n = 5 for uninfected cells. Using an unpaired t test the Δmax of UL31 in uninfected HeLa/EGFP31 cells was compared to the Δmax of HSV-2 infected HeLa/EGFP31 cells (*p < 0.05). (C) Images of a live HeLa/EGFP31 cell on a grid-bottom dish and infected with HSV-2 for 5 hours. Images taken immediately before (Pre), and immediately after (Post) the indicated area (white arrowhead) was microirradiated with a 405 nm laser using an Olympus FV1000 laser-scanning confocal microscope. Cells were immediately fixed and stained with anti-UL31 antibody followed by staining with Alexa 568 conjugated donkey anti-chicken secondary antibody. Microirradiated cells were relocated and images captured by confocal microscopy (Fixed Cells). Image is representative of n = 8 cells analyzed in two independent experiments.

Back to article page