Figure 2 | Scientific Reports

Figure 2

From: The Herpesvirus Nuclear Egress Complex Component, UL31, Can Be Recruited to Sites of DNA Damage Through Poly-ADP Ribose Binding

Figure 2The alternative text for this image may have been generated using AI.

Recruitment of UL31 to sites of laser microirradiation requires its N-terminal 45 amino acids but not CR1. (A) Cartoon of HSV-2 UL31 identifying key features of the molecule including the position of the bipartite nuclear localization signal (NLS) as well as the positions of conserved regions (CR) 1 through 4. (B) Images of live HeLa cells expressing wild type EGFP-UL31 (WT), EGFP-UL31 lacking the CR1 region required for UL34 binding (ΔCR1), or EGFP-UL31 lacking its N-terminal 45 amino acids (Δ45) acquired immediately before (Pre), immediately after (Post), and 30 seconds after the areas indicated by arrowheads were microirradiated using an Olympus FV1000 laser-scanning confocal microscope. The graphs display quantitation of the average fluorescence intensity of the irradiated area (Bleach), as well as distal control areas (Control), over time. Images shown are representative of n = 12 cells analyzed in two independent experiments. Error bars are standard error of the mean (n = 12 per condition).

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