Table 1 Primers used to construct UL31 expression plasmids.
Plasmid | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Template |
|---|---|---|---|
EGFP-UL31 | AGTTCGAATTCTATGTATGACATCGCCCCCCGTCGC | GATCGTCGACCTACGGCGGAGGAAACTCGTCG | HSV-2 HG52 vDNA |
Δ11 | GATCGAATTCTCGGCCCGGGCCCGGCCGCGACAAG | GATCGTCGACCTACGGCGGAGGAAACTCGTCG | EGFP-UL31 |
Δ20 | GATCGAATTCTCGGCGGCG GTCGCGCTTTTCC | GATCGTCGACCTACGGCGGAGGAAACTCGTCG | EGFP-UL31 |
Δ23 | GATCGAATTCTTCGCGCTTT TCCGCCGCCGGGAACC | GATCGTCGACCTACGGCGGAGGAAACTCGTCG | EGFP-UL31 |
Δ27 | GATCGAATTCTGCCGCCGG GAACCCCGGCGTG | GATCGTCGACCTACGGCGGAGGAAACTCGTCG | EGFP-UL31 |
Δ45 | GACTGAATTCTCACGCGCGCAGGCTGGAGCTCTGCCTGCACG | GATCGTCGACCTACGGCGGAGGAAACTCGTCG | EGFP-UL31 |
N63 | AGTTCGAATTCTATGTATGACATCGCCCCCCGTCGC | GATCGTCGACCTAGAAGCCGCGGTAGCGACG | EGFP-UL31 |
12–63 | GCAGAGCTGGTTTAGTGAACC | GATCGTCGACCTAGAAGCCGCGGTAGCGACG | Δ11 |
21–63 | GATCGAATTCTCGGCGGCGGTCGCGCTTTTCC | GATCGTCGACCTAGAAGCCGCGGTAGCGACG | Δ20 |
12–54 | GCAGAGCTGGTTTAGTGAACC | GATCGGTACCTTACAGGCAGAGCTCCAGCCTGC | Δ11 |
21–54 | GATCGAATTCTCGGCGGCGGTCGCGCTTTTCC | GATCGGTACCTTACAGGCAGAGCTCCAGCCTGC | Δ20 |
ΔCR1 5′ | AGTTCGAATTCTATGTATGACATCGCCCCCCGTCGC | GGCGCGGTAGCGACGGCG CTCGTGCAG | EGFP-UL31 |
ΔCR1 3′ | CCTGCACGAGCGCCGTCGCTACCGCGGCAGCGCGGGCGACGGGCGGCTGGCCACC | GATCGTCGACCTACGGC GGAGGAAACTCGTCG | EGFP-UL31 |
HCMV UL53 | GATCGAATTCTATGTCTAGCGTGAGCGGCGTGC | GATCGGTACCTCAGGGCGCACGAATGCTCTTG | HCMV vDNA |
KSHV ORF69 | AGTTCGAATTCTATGCC GAAATCAGTGTCCAGCC | GATCGTCGACTTATAGGGCGTTGACAAGTGC | KSHV iSLK.219 infected cells |
