Figure 2

LLC cell-conditioned medium activates TLR4 in reporter cells. TLR4 reporter cell line HEK-Blue hTLR4 (InvivoGen) was treated with LCM or control medium for 24 h and TLR4 activation was measured as the enzymatic activity of secreted embryonic alkaline phosphatase (SEAP) in arbitrary unit (a.u.). Data was analyzed by Student t test. *Denotes a difference (P < 0.05).