Figure 3

Droplet bioassay. (A) droplet feeding bioassay showing a pair of neonate EAB larvae feeding on a 2 uL drop of blue sucrose solution containing 6 ug/uL dsRNA and the 1μL pipette tip as scale; (B) Starting point of feeding assay and dsRNA exposure, 2 larvae per drop; (C) EAB larvae after feeding, showing intestinal tract with blue color; (D) Comparison between droplet fed larva (blue) and un-fed larva. Olympus SZ61 Stereo Zoom Microscopy was used to amplify the EAB larvae and the pictures were taken by Olympus DP12 Microscope Digital Camera and iPhone 6S.