Figure 1 | Scientific Reports

Figure 1

From: Improved efficacy and in vivo cellular properties of human embryonic stem cell derivative in a preclinical model of bladder pain syndrome

Figure 1The alternative text for this image may have been generated using AI.

Isolation and characterization of hESC-derived M-MSCs. (a) Experimental scheme for the transwell-based differentiation. (b) Morphological characterization of M-MSCs. Scale bar = 200 μm. (c) M-MSCs at passage of 8 were analyzed for specific surface antigen marker expression for hMSCs (CD44, CD90, and CD73), pericytes (PDGFRB, CD146, and NG2), endothelial cells (KDR and Tie-2), hematopoietic progenitors (CD133), and MHC class I (HLA-ABC) and II (HLA-DR). (d) Karyotypic analysis of M-MSCs at passage 19. The isolated cells were capable of stable proliferation without chromosomal changes. (e) (i) Differentiation potential of M-MSCs was shown by adipogenesis (left, Oil red staining, scale bar = 50 μm) and osteogenesis (right, Alizarin red staining, scale bar = 500 μm). (ii) In vitro tube assembly assay. Scale bar = 500 μm. (f) Dye transfer (circle in the plot) increased in a coculture of DiI-labeled M-MSCs (M-MSC-DiI, red) and calcein-labeled human umbilical vein endothelial cells (HUVEC-Calcein, green). Dye-transfer between M-MSCs and HUVECs was visualized by flourescence microscopy (white arrowheads).

Back to article page