Figure 3 | Scientific Reports

Figure 3

From: Improved efficacy and in vivo cellular properties of human embryonic stem cell derivative in a preclinical model of bladder pain syndrome

Figure 3The alternative text for this image may have been generated using AI.

Histological analysis of M-MSC injection effects on HCl-induced bladder injury. (a) The immunostaining of cytokeratin (i) (magnification ×40, scale bar = 100 μm), Toluidine blue (ii) (magnification ×200, scale bar = 100 μm), Masson’s trichrome staining (iii) (magnification ×100, scale bar = 100 μm), and TUNEL assay (iv) (magnification ×400, scale bar = 100 μm) in bladder tissues of HCl-IC rats 1 week after injection of PBS vehicle or M-MSCs at the indicated dosage (K = a thousand). Arrows indicate infiltrated mast cells. Sham: sham-operated. Nuclei were stained with Mayer’s hematoxylin (i, ii, and iii) or DAPI (blue, iv). Arrows (ii) indicate infilated mast cells. (b) Quantification of histological examniations. Data were normalized to the sham group (n = 15). Data are presented as the mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 compared to the HCl-IC group; #p < 0.05, ##p < 0.001, ###p < 0.001 compared to the 1000 K group with Bonferroni post-test.

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