Figure 5 | Scientific Reports

Figure 5

From: Improved efficacy and in vivo cellular properties of human embryonic stem cell derivative in a preclinical model of bladder pain syndrome

Figure 5The alternative text for this image may have been generated using AI.

Central role of Wnt and IGF signaling activity on therapeutic effect of M-MSCs. (a,b) RQ-PCR analyses of expression levels of Shh, Wnt, and downstream growth factors (a) and representative confocal micrographs (magnification ×1,000, scale bar = 10 μm) for immunofluorescence staining of β-catenin protein (b) in the bladder tissues of the indicated groups 1 week after the injection of 1 × 106 M-MSCs or PBS vehicle into HCl-IC animals. Expression is presented as % Gapdh (n = 10). (c) Quantitative data of bladder voiding parameters in rats (from 8 independent animals per group) at 1 week after the injection of 1 × 106 M-MSCs into HCl-IC animals in the absence or presence of indomethacin (Wnt blocker) or Gefitinib (IGF-mediated signaling inhibitor) intervention. All data are presented as the mean ± SEM, **p < 0.01, ***p < 0.001 compared to the HCl-IC group with Bonferroni post-test. (d) Detection of M-MSCs stably expressing GFP (red) in the indicated rat bladders by immuostaining (magnification ×200, scale bar = 200 μm). Nuclei were stained with DAPI (blue). Arrow heads indicate the engrafted cells. U; Urothelium, S; Serosa.

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