Figure 4
From: Targeting melanoma stem cells with the Vitamin E derivative δ-tocotrienol
Intracellular autofluorescence, associated with ABCG2 expression, is a novel marker for A375 CSCs. (a) Cellular autofluorescence (λem 532 nm) was measured in A375 adherent and spheroid-derived cells by flow cytometry, observing the presence of two cell populations. Data were analyzed by unpaired t-test, and are represented as mean values ± SEM (*p < 0.05 vs Adherent cells). One representative of four different experiments is shown. (b) The proportion of ABCG2-positive cells was measured in adherent or melanosphere-derived cells. Gating strategy was based on negative controls, without incubation with the specific antibody. Data were analyzed by unpaired t-test and are represented by mean values ± SEM (*p < 0.05 vs Adherent cells). One representative of four different experiments is shown. (c) The relationship between autofluorescence and ABCG2 expression in melanosphere-derived cells was analyzed by flow cytometry. Gating strategy was based on negative controls, without incubation with the specific antibody (left panel). Moreover, highly autofluorescent cells were gated and analyzed for their ABCG2 positivity (right panel). One representative of three different experiments is shown.
