Figure 5
Detection of horizontal gene transfer (HGT) events for GH30 genes in fungi. (A) The Maximum likelihood phylogeny of 50 genes (bootstrap 1000) based on GH30 domain sequences annotated by Pfam PF14587 or PF02055 family. The best model of WAG + I + G + F was identified by ProtTest analysis. The first group (c-1) is comprised of plant-parasitic, endophytic, and mycoparasitic fungi (Trichoderma spp., Fusarium spp., and C. purpurea), and the two nematode-trapping fungi (M. haptotylum and A. oligospora). The two duplicated gene pairs of P. chlamydosporia are separately attributed to the other two groups (c-2 and c-3). The VFPPC_01957-VFPPC_02227 pair clusters with different lifestyle fungi (c-3), including plant parasites (F. oxysporum and C. purpurea), the nematode egg parasite P. lilacinum, the insect parasite T. hemipterigena, endophytic and mycoparasitic fungi (Trichoderma spp.), and the nematode-trapping fungus M. haptotylum. The VFPPC_07807-VFPPC_09315 pair clusters with nematode pathogens (P. lilacinum and H. minnesotensis), insect pathogens (T. hemipterigena and T. inflatum), and endoparasites and mycoparasites (Trichoderma spp. and T. ophioglossoides). (B) The phylogeny of 2,786 VFPPC_07807 homologous genes. The two clades (BF-D1 and BF-D2) contain Ascomycota and Basidiomycota genes, respectively. In BF-D1, 118 fungal genes are clustered with six bacteria genes, and another sub-clade contains four fungal genes and one bacteria gene. (C) Distribution of 2,786 genes, including 2,237 (80.29%) bacteria genes. (D) Phylogenetic topology of eukaryotes and bacteria. The topology is inferred from the global tree in study by Burki88 and topologies provided by Tree of Life Web Project (http://tolweb.org/tree/phylogeny.html).
