Figure 4 | Scientific Reports

Figure 4

From: Whole-exome sequencing identifies two novel mutations in KCNQ4 in individuals with nonsyndromic hearing loss

Figure 4The alternative text for this image may have been generated using AI.

Dominant-negative effects of the mutant KCNQ4 channels. (a) Individual mutants (p.D266Y and p.V87_N89del) were co-expressed with WT KCNQ4 at the indicated WT:mutant cDNA molar ratios, and linopirdine-sensitive K+ current traces were recorded at +40 mV. Dashed lines indicate zero current levels. (b) Comparison of current densities at +40 mV. WT:mutant cDNA ratios are indicated under the bar graphs, and the total amount of cDNA was equalized in all groups by adding empty vector (pRK5). (c) Suppression of WT-mediated current by the co-expression of mutant (Mt) KCNQ4 channels. The mean values of the current densities obtained at +40 mV were normalized, and the current suppression ratios were denoted against WT/(WT + mutant) ratios of cDNA transfected. Dashed line with a square symbol denotes the predicted suppression ratio expected for the tetrameric channel. Mean ± SEMs (n = 10–24).

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