Figure 4 | Scientific Reports

Figure 4

From: Structural biology workflow for the expression and characterization of functional human sodium glucose transporter type 1 in Pichia pastoris

Figure 4The alternative text for this image may have been generated using AI.

Detergent screening. (A) Percentage of fluorescence of solubilized membranes compared to lysate total fluorescence after solubilization with different detergents. Samples were read in a plate reader with a combination of excitation and emission filters of 485/520 nm, respectively. UDM (n-Undecyl-β-D-Maltopyranoside), SB3-12 (Sulfobetaine 3-12), OG (Octyl-β-Glucoside), NDM (n-Nonyl-β-D-Maltopyranoside), MEGA 8, 9, 10 (N-Octanoyl, N-nonanoyl, N-decadyol)-(N-Methylglucamine), LDAO (N,N-Dimethyldodecylamine N-Oxide), LAPAO (3-Dodecylamido-N, N′-Dimethylpropyl Amine Oxide), DMNG (Decyl Maltose Neopentyl Glycol), DDAO (Decyldimethylamine-N-Oxide), DM (Decyl-β-D-Maltopyranoside), DDM (Dodecyl-β-D-Maltoside), Cymal 7 (7-Cyclohexyl-1-Heptyl-β-D-Maltoside), Cymal 6 (6-Cyclohexyl-1-Hexyl-β-D-Maltoside), Cymal 5 (5-Cyclohexyl-1-Pentyl-β-D-Maltoside), C12E5 (Dodecyl Pentaethylene Glycol Ether), C12E8 (Octaethylene Glycol Monododecyl Ether), C12E9 (Dodecyl Nonaethylene Glycol Ether), C8E5 (Tetraethylene Glycol Monooctyl Ether), C8E5 (Octyl Pentaethylene Glycol Ether), CHAPS (3-[(3-Cholamidopropyl) Dimethylammonio]-1-Propanesulfonate), CHAPSO (3-[(3-Cholamidopropyl) Dimethylammonio]-2-Hydroxy-1-Propanesulfonate), F10 (n-Decylphosphocholine), F11 (n-Undecylphosphocholine), F12 (n-Dodecylphosphocholine). (B) FSEC chromatogram in a Superdex 200 HR column of membranes solubilized for 2 hours with selected detergents at 1% (w/v) concentration.

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