Figure 1

IBA-1⁺ macrophages were increased in lesional human Ps skin. IBA-1 IHC staining of non-lesional and lesional skin of the Ps patients (n = 4) (a) representative images of IHC staining of IBA-1 (red) in epidermis and dermis (black filled triangle) area. Inset image represents active parakeratosis area within the lesional skin biopsy. IBA-1⁺ macrophages resided in both epidermis and dermis area and congregated under the active parakeratosis region. Section of whole skin biopsies of both lesional and non-lesional were scanned with digital scanner at 20X objective. The representative images within the skin section were from 8X digital magnification. Inset area of the lesional skin was from 32X digital magnification. (b) quantification of IBA-1⁺ signal of non-lesional and lesional skin of Ps patients. IBA1⁺ staining was normalized to the length of biopsy epidermis analyzed for each donor and presented as area (μm²) divided by length (μm). Data are shown as mean ± SEM.; *P < 0.05.