Figure 6

Two distinct Mon/Mac (Ki67⁺ and TNFα⁺) subsets increased in IL-23 inflamed skin. (a) Representative 2-dimentional couture plot of TNFα and Ki67 expression in Mon/Mac cells from IL-23 injected ears at day 4 determined by flow cytometry. The quad gate that separates TNFα and Ki67 positive/negative was determined based on comparing fluorescent staining from IL-23 ears to those from naive ears and IL-23 injected ears without the specific fluorescent antibody. Numbers indicate the frequencies of the cell population within the quad gates. (b) Enumeration of Ki67⁺ and TNFα⁺ Mon/Mac cells in ears of sham and IL-23 injected animals (n = 6–8). Frequencies of Ki67⁺ and TNFα⁺ Mon/Mac cells were determined as described in (a) and were used to calculate the number of cells per ear. Data are shown as mean ± SEM. **P < 0.01.