Figure 3
Effect of FGF18 on astrocyte cultures. (a) FGF18 treatment robustly increases GLT1 levels in astrocyte cultures. Images are representative of n = 3 independent experiments. Scale bar = 50 μm. (b) Western blot of mESC-derived astrocyte cultures prior to and following treatment or not with FGF18. GLT1 and GFAP are increased and decreased, respectively, in cultures treated for 6 days with FGF18, compared to non-treated astrocyte cultures of the same age. Actin protein was used as loading control (blots are representative of three independent experiments). (c) Heat map profiles for gene expression. The panel to the left shows all genes, while that to the right is for genes with at least four-fold difference up or down. (d) List of top 20 genes up (red bars) and down (blue bars) regulated with FGF18 treatment. (e) Venn diagram showing overlap of genes with 4-fold expression change between the D6 NT vs D0 and D6 FGF2 vs D0. (f) List of annotated genes common to FGF2- and FGF18-treated astrocyte cultures. (g) Significantly enriched GO Slim terms in the Reactome pathways for genes with over 1.5-fold expression change. (h) Heat map representing the expression of astrocyte-specific genes54 following treatment with FGF2 and FGF18 (blue and red colored squares show low and high expression, respectively, of genes of interest). Statistically significant changes are marked with an asterisk.
