Figure 4 | Scientific Reports

Figure 4

From: FGF family members differentially regulate maturation and proliferation of stem cell-derived astrocytes

Figure 4The alternative text for this image may have been generated using AI.

Effect of FGF18 on astrocyte cultures. (a) Heat map representing the expression of pan-reactive, A1- and A2-specific genes, for each analyzed condition. Data are represented as fold change up (red colored squares) or down (blue colored squares). Significant changes are marked with an asterisk. (b) Quantification of the total number of Hb9::GFP MNs and the mean outgrowth per MN after 2 days in co-culture with astrocytes treated with FGF18, FGF2, FBS, BMP4, CNTF or non-treated. Mean ± SEM; n = 3 independent experiments. P values: **P < 0.01; ****P < 0.0001. Scale bar = 50 μm. (c) Representative images of Hb9::GFP MNs after 2 days in co-culture with astrocytes treated with FGF18, FGF2, FBS, BMP4, CNTF and non-treated astrocytes. (d) Summary of our findings; FGF2, FGF4, FGF6, FGF9, FGF16 and FGF18 trigger GLT1 expression; FGF2, FGF4, FGF6 and FGF9 also promoted astrocyte growth. BMP4 triggers GFAP expression; BMP4-treated astrocytes failed to support the survival of MNs.

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