Figure 2
From: Role of p53 isoforms in the DNA damage response during Drosophila oogenesis
Genes involved in germline cell-death by IR and poor nutrition. (a) Females were irradiated at 3 Gy and TUNEL staining was performed with the ovaries 6 h after irradiation. The percentages of TUNEL-positive germline cells in the germarium region 2 in lok, p53, hid, and rpr mutant females were determined. The values are the mean ± SD of three independent experiments (ns p > 0.05, *p < 0.05, ***p < 0.001). At least 200 germarium in total were counted for each genotype. (b) Expression pattern of the hid transcript after irradiation. Wild type and p535A-1-4 mutant females were irradiated at 40 Gy and RNA fluorescent in situ hybridization and protein immunofluorescence double labeling was performed using Dig-labeled anti-sense hid RNA as a probe and antibody against Vasa. (c) Wild type and mutant females were grown in rich or poor nutrition conditions and TUNEL staining of the ovaries was performed. The percentages of ovarioles containing TUNEL-positive germline cells in the germarium region 2 (left panel) and TUNEL-positive egg chambers of stage 7–10 are shown (right panel). The values are the mean ± SD of at least two independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001). At least 235 ovarioles in total were counted for each genotype. (d) Wild type and p535A-1-4 mutant females were irradiated with 40 Gy and ovarioles containing TUNEL-positive germline cells in the germarium region 2 or stage 7–10 egg chambers were counted at 6, 24, 48, 72, and 96 h after irradiation. The values are the mean ± SD of at least two independent experiments. At least 100 ovarioles in total were counted for each timepoint.
