Figure 1 | Scientific Reports

Figure 1

From: Covalently modified carboxyl side chains on cell surface leads to a novel method toward topology analysis of transmembrane proteins

Figure 1The alternative text for this image may have been generated using AI.

The reaction flow of the applied labelling method. The carboxyl amino acid side chains of Bovine Serum Albumin model protein were activated by EDC and Sulfo-NHS, and then biotinylated by Biotinyl Cystamine. During the nanoLC-MS/MS sample preparation, the disulphide bridges were cleaved by DTT and alkylated by Iodoacetamide reagents.

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