Table 2 Labeling and stability of [68Ga]Ga-(HE)3-ZHER3-DOTA, [68Ga]Ga-(HE)3-ZHER3-DOTAGA, [68Ga]Ga-(HE)3-ZHER3-NODAGA. Stability of the radiolabeled complexes is expressed as % release (n = 6).

From: Increase in negative charge of 68Ga/chelator complex reduces unspecific hepatic uptake but does not improve imaging properties of HER3-targeting affibody molecules

Analysis

[68Ga]Ga-(HE)3-ZHER3-NODAGA (n = 3)

[68Ga]Ga-(HE)3-ZHER3-DOTA (n = 3)

[68Ga]Ga-(HE)3-ZHER3-DOTAGA (n = 5)

Radiochemical yield (%), % Release of 68Ga in EDTA challenge (1000× EDTA, 10 min, 85 °C)

97 ± 2*

53 ± 30

81 ± 16

7 ± 2

11 ± 6

Purity after NAP5 size exclusion (%)

<99*

99.7 ± 0.5

99.8 ± 0.2

% release in PBS, 1 hour

0.03 ± 0.05*

0.4 ± 0.2

0.3 ± 0.4

% release in human serum, 1 hour 37 °C

0.23 ± 0.05*

3.7 ± 0.7

1 ± 1

  1. *Data for [68Ga]Ga-(HE)3-ZHER3-NODAGA was previously reported by40.
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