Figure 1

Morphological and Functional Characterization of Human Hepatocyte stem-like cells, HepCY and HepCO. (A) Photomicrographs showing phase contrast microscopy of morphological studies of HepCY from passage 1 to passage 6, Objective magnification: 10X upper panel (Scale Bar: 100 µm), 20X lower panel (Scale Bar: 50 µm). (B) Phase-contrast photomicrographs showing morphologic changes of passage 8 HepCO (I) and high passage HepCY (II) & HepCO (III) toward the hepatocyte phenotype, Objective magnification: 10X upper panel (Scale Bar: 100 µm), 20X lower panel (Scale Bar: 50 µm); (C) The level of gene expression in different passages, HepCY cells and passage 8 HepCO cells determined by RT-PCR: (I) Hepatocyte-specific & related protein, (II) The expression levels of Tumor protein TP53, TP73 and PRDM16, MEGF6, CEP104 and DFFB genes proximal to the TP73 gene locus in chromosome1p36.32 region. Note: The row of bands representing the expression of each gene and separated by white spaces as shown in the gels displayed in panel C-I and C-II are cropped from full-length gels of the corresponding genes. The same exposures were made for each gel. The original gels for each figure are shown in the Supplementary Information File.