Figure 4

Abnormal positioning of leukocytes in tissues of TIPE-deficient mice under the steady state. (a) The total white blood cell, lymphocyte, and neutrophil counts of the blood of normal 6–8-week-old WT (n = 37), Tnfaip8^−/− (n = 10), Tipe2^−/− (n = 15), and DKO (n = 35) mice. (b) The numbers of the indicated cell subsets of thymus of WT (n = 6) and DKO (n = 8) mice as determined by flow cytometry. CD4⁺ and CD8⁺ denote the respective single positive cells; DP denotes double positive cells; DN denotes double negative cells. (c) The numbers of the indicated cell subsets of spleen of WT (n = 6) and DKO (n = 8) mice as determined by flow cytometry. (d) Relative numbers of the indicated intraepithelial cells per mg of small intestine of WT (n = 10 mice for CD45⁺ and 9 for CD3⁺ cells) and DKO (n = 13 for CD45⁺ and 8 for CD3⁺ cells) mice as determined by flow cytometry. Data are normalized to the mean of the respective WT group. The values are mean ± s.e.m. (a–d), and are pooled from two (b,c) or three (a,d) independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test (a–d)).