Figure 5

Abnormal positioning of leukocytes in the nervous tissue of TIPE-deficient mice during encephalomyelitis. (a) EAE scores of Rag2^−/− mice (n = 4 mice per group) that were injected with MOG-specific WT or DKO CD4⁺ T cells. (b) The percentages of WT CD45.1⁺ and DKO CD45.2⁺ T cells among total CD3⁺ T cells in the blood (BLD) and spinal cord (Sp Cord) of EAE mice (n = 7 mice per group) that were injected with WT and DKO bone marrow cells at 1:1 ratio. Samples were collected one day after EAE onset. (c) The percentages of transferred CMTMR-labeled WT and CMFDA-labeled DKO cells among total leukocytes in the blood (BLD), spleen (SPL), and spinal cord (Sp Cord) of EAE mice (n = 5 mice per group) that were injected with the respective MOG-specific WT and DKO CD4⁺ T cells at 1:1 ratio. Samples were collected on the day of the EAE onset. The values are mean ± s.e.m. (a–c), and are pooled from two independent experiments (b,c) or are representative of four independent experiments (a). *P < 0.05; **P < 0.01; ****P < 0.0001 (Mann-Whitney U test (a,b) or Student’s t-test (c)).