Table 1 PacBio Iso-seq output statistics.

From: Full-length transcript sequencing accelerates the transcriptome research of Gymnocypris namensis, an iconic fish of the Tibetan Plateau

Libraries

09061

09062

Polymerase reads

492,350

520,123

Mean length of polymerase reads

17,327

17,542

Polymerase reads N50

34,250

33,250

Subreads

5,790,289

6,032,472

Mean length of subreads

1,396

1,434

Number of circular consensus sequence reads (CCS)

295,248

320,626

Mean length of CCSs

1,663

1,690

Total bases of CCSs

491,225,617

542,141,219

Number of reads with 5′ adapter sequence

282,632

308,526

Number of reads with 3′ adapter sequence

284,198

309,794

Number of poly-A reads

281,763

307,616

Number of full-length reads

270,520

296,536

Number of full-length non-chimeric reads

267,490

292,946

Mean full-length non-chimeric read length

1,542

1,568

Full-length percentage (FL%)

54.94

57.01

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