Figure 3 | Scientific Reports

Figure 3

From: Marine alkaloid monanchoxymycalin C: a new specific activator of JNK1/2 kinase with anticancer properties

Figure 3The alternative text for this image may have been generated using AI.

Functional kinome profiling of serine/threonine kinases (STK) in 22Rv1 cell treated with MomC for 2 h. Proteins were extracted and analyzed using STK-PamChip® (sequence-specific peptide phosphorylation assay) and anti-phospho-STK antibodies (a). Heat-map plot (b), box plot (c), or volcano-plot (d) were constructed. Red squares indicate substrate peptides which have significantly higher phosphorylation (log2(p) > 1.3, dotted line) compared to control (d). Upstream analysis of kinases affected under the treatment (e). Normalized kinase statistic > 0: higher kinase activity in MomC-treated cells; specificity score > 1.3 (white to red bars): statistically significant changes (e).

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