Figure 2

Multiple sequence alignment of EsNap with other closely related plant napins 2SS3_BRANA (Napin-3: Brassica napus), 2SSE_BRANA (Napin embryo-specific: Brassica napus) and Nap_ ERUSA (Napin: Eruca sativa). Secondary structure elements (α-helices and β-sheets) of EsNap are indicated at the top. Signal peptide, N/C terminus and turns are indicated with green, yellow and black bars respectively. Identically conserved residues are labeled by asterisks (*), while semi-conserved substitutions are labeled by single dots (.) and cysteine involved in disulfide bonds between two chains are shown in red color. The highly flexible region is highlighted with a red box. Smaller and larger chains are shown in dot line (–) upper on the secondary structure elements in green and red color respectively Multiple sequences alignment was performed by using ClustalW in the default set up and BoxShade server.