Figure 1

Gene arrangement and read depth along reconstructed ancient mitochondrial genomes. All aDNA reads were mapped in BWA v.0.7.17 to the Philolithus actuosus TB20958 (Tenebrionidae: Asidini) mitogenome. Asterisk indicates samples containing > 1% ambiguous sequences from lack of coverage. Red lines mark average coverage. White fields represent control (CR) and other non-coding regions (nc). Color codes: Red: C to T substitutions; Blue: G to A substitutions; Grey: All other substitutions; Orange: Soft-clipped bases; Green: Deletions relative to the reference; Purple: Insertions relative to the reference. Subfossil fragments (Philolithus actuosus) used in the extraction process were illustrated for 34,355 ybp sample.