Table 1 Summary of ancient samples reported in this study.

From: Recovery and analysis of ancient beetle DNA from subfossil packrat middens using high-throughput sequencing

Calibrated age midpoint (ybp)

Original sample code

Origin

Available/used* body fragments

DNA extract concentration (ng/μl)

Library concentration (ng/μl)

Amount used for lib. prep

Total # of recovered reads

# of aligned genomica reads

# of aligned mitochondrialb reads

1615

5323

Joshua Tree National Park (JTNP) (Jumbo Rock)

Pronotum, coxa + femur + tibia*

0.558

10.9

Whole extract (20 µl)

2,252,684

3511 (0.156%)

6777

2035

7423

JTNP (Jumbo Rocks North)

Femur + tibia*

1.06

16.2

11 µl

2,262,666

3973 (0.176%)

12,073

8480

80B23

JTNP (Hidden Valley)

Femur + tibia*

NM

12.9

Whole extract (20 µl)

2,386,019

712 (0.030%)

601

16,610

41B24

Sierra Juarez (Guadalupe Canyon)

Femur + tibia*

0.548

19.3

Whole extract (20 µl)

–

–

–

34,355

60B23

JTNP (Indian Cove)

Abdominal ventrites, pronotum, femur + tibia* (3 sets)

1.99

10.5

 

2,471,490

133 (0.005%)

45

–

Ex Blk1

–

–

NM

3.34

Whole extract (20 µl)

473,099

0

1

–

Libr Blk

–

–

–

2.88

–

292,658

0

–

  1. *Body part used for extraction; + intact body parts; NM not measurable; Ex and Libr Blk extraction and library blanks; aEndogenous genomic DNA content measured as the total number of recovered reads mapped to the Tribolium castaneum genome; bNumber of reads mapped to the reference mitogenome of Philolithus actuosus.
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