Figure 3
Histology shows iron-containing Aβ plaques throughout the brain in 5xFAD mice and TgF344-AD rats. (a) 48G anti-amyloid staining confirmed the presence of plaques not only in the hippocampus, but in the deep cortical layers and subcortical regions, including the thalamus, of 5xFAD mice. (b) The plaques visible on MRI were mostly limited to the hippocampus, with a few cortical plaques visible in a few mice. The contrast in these MGE images is largely T2*/susceptibility-driven. Perl’s-DAB staining showed that while many of the plaques stained with 4G8 contained iron, the largest iron deposits were in the dorsal hippocampus (c, d). (e) 48G anti-amyloid staining confirmed the presence of plaques throughout the hippocampus and cortex of TgF344-AD rats. (f) Hippocampal and cortical plaques were visible on MRI. Susceptibility artifacts around the air-tissue interfaces at the ventral surface of the brain obscured visualization of plaques in those areas. (g) Perl’s-DAB staining showed a spatial distribution of iron very similar to Aβ. All images were acquired from one wild-type mouse (a—left), one 5xFAD mouse (a—right, b, c), one wild-type rat (e—left), and one TgF344-AD rat (e—right, f, g). Scale bars in (a) and (e) = 500 microns.
