Figure 4

In vivo QSM and Alizarin Red staining reveal thalamic calcifications in 5xFAD mice. (a, b) Quantitative susceptibility (χ) maps of a 5xFAD mouse (a) before (pre-Mn) and (b) after administration of MnCl2 (Post-Mn). Bilateral clusters in the thalamus were diamagnetic Pre-Mn but became paramagnetic Post-Mn (arrows). (c) A section from the same mouse, stained with Alizarin Red, shows that these thalamic clusters contain calcium and/or manganese. (d) An Alizarin-Red-stained section from a 5xFAD mouse without MR-visible thalamic clusters. Inset scale bars = 100 μm.