Figure 3
From: Non-covalent SARS-CoV-2 Mpro inhibitors developed from in silico screen hits
Compound Z222979552 reduces SARS-CoV-2 replication in Vero E6 cells in a dose-dependent manner. (A) SARS-CoV-2 titers upon treatment with compound Z222979552 or DMSO control. Viral titers are displayed as fifty-percent tissue culture infective dose (TCID50)/ml 24 h post-infection. TCID50 values correspond to the viral titers (log10 scale) required to kill fifty-percent of infected host cells. The experiment was performed in triplicate and the results are shown as means and standard deviations. (B) Immunofluorescence staining of infected Vero E6 cells for double-stranded RNA (dsRNA). The cells were treated with compound Z222979552 or DMSO and were stained 24 h post-infection. Green, dsRNA; blue, DAPI. One representative image out of three biological replicates is shown. (C) Z222979552-mediated cytotoxicity, determined using Vero E6 cells treated with the compound or DMSO for 24 h. The experiment was performed in triplicate and the results are shown as means and standard deviations.
