Figure 1

Ether inhibits hair podium [Ca]_ext spread into the trap. (A) Representative time courses of the fluorescence intensity measured in the trigger hair podium (black) and in the trap (blue) as seen in Supplementary Movie S1. First subthreshold stimulus only was detected in the hair (image 1). The second suprathreshold stimulation occurred first in the trap podium (image 2) and then spread to the trap tissue (image3) before returning to baseline (image 4). Decay curves of fluorescence intensities were best fitted using a double exponential function (dotted red line) with the given tau values of 4 different traps (mean ± SD; n = 4). (B) Timeline of the ether anesthesia of a trap. The traps were mechanically stimulated on the trigger hair every minute. At timepoint 0, a saturated ether atmosphere was added for 5 min. After ventilation with fresh air, first Ca²⁺ signals were measured after 12 min again (mean ± SD (shading); n = 4). Below are sample images from the Supplementary Movie S3 of the indicated timepoints.