Figure 2

Porcine skin samples were imaged with a PS-OCT system after burn induction. OCT intensity images are shown for a representative cross-sectional view (a) and superficial en face view (b) for an ROI spanning a tissue region subject to 30 s burn duration (left side of each panel), no burn (center of each panel), and 60 s burn duration (right side of each panel). The degree of polarization (DOP) was spatially mapped throughout each cross-section (c). The surface of each tissue section was identified automatically and a DOP threshold was applied to determine the depth extent of the injured collagen in each cross section (white dashed lines). The difference between these two lines [e.g., orange arrow in (c)], adjusted by tissue refractive index, was used to calculate the collagen injury depth for each cross section of the imaged volume to produce an en face map of DOP-based collagen injury depth (d) corresponding to the same ROI as (b). Scale bars = 1 mm. Intensity limits for 2D colorbars in (c) and (d) range from 0 dB to 50 dB.