Figure 4
From: Bioinspired gelatin based sticky hydrogel for diverse surfaces in burn wound care
In vitro and in vivo performance of GbH as a dressing material for second-degree burn wounds: Cytotoxicity assessment of the GbH using L929 cells: (a) Cell viability of L929 when exposed to GbH for 24 h, where Hb is hydrogel base with no stabilizer and the legend denotes the concentration (mg) of each test stabilizer; (b) Cell viability of 3T6 when exposed to the GbH leachate medium for 24 h, where Ctr is the GbH base with no stabilizer and the legend denotes the ratio of polydopamine to sodium metaperiodate and (c) Cell viability of 3T6 when exposed to the GbH leachate media for 24 h at a different percentage, where Ctr is the GbH base with no stabilizer and the legend of the ratio denotes polydopamine and sodium metaperiodate ratio in the GbH. (d) Cell viability of the HaCat when the GbH is in contact with the cells (GbH placed) and leachate medium at 100% medium replacement incubated for 24 h, where Ctr denotes control, Ctr 37 °C denotes the positive control; (d.1) Observed event of healthy HaCat cells after 24 h in contact with the GbH, where white arrow denotes the GbH and black arrow denotes healthy HaCat cells in 96-well plate. Observed development of brine shrimp when exposed to the GbH for 24 h, (e) Positive control: nauplii from the aerated tank; (e.1) Control: nauplii in artificial saltwater; (e.2) and (e.4) Test: nauplii in 1:1 medium of the GbH leeched buffer and artificial saltwater; (e.3) and (e.5) Test: The GbH placed in brine shrimp in an artificial saltwater medium. Short–term toxicity test on embryo and sac-fry stages of zebrafish model: (f) Observed development of zebrafish when exposed to the GbH (GbH direct contact and 1:1 medium, i.e., the GbH leeched buffer and E3 medium) for 18–96 hpf; (f.1) Observed healthy zebrafish eggs at 48 hpf in contact with the GbH. Photomicrographs showing the histopathology of the skin in rats. Normal appearances of the epithelium in the male (g) and female (g.1) rats of the control group. Minimal acanthosis of the male (g.3) and female (g.4) rats. Normal dermis appearance in the control rat (g.2) and minimal inflammatory cell infiltration in the treated (g.5) group.
