Figure 7

Protein–protein interactions of PtoCYCD2;1 with PtoCDKs. Yeast cells were co-transformed with pGBKT7 and pGADT7 constructs carrying the corresponding genes and grown on SD-Trp-Leu, SD-Leu-Trp-His + 10 mM 3-AT, SD-Leu-Trp-His + 20 mM 3-AT, SD-Leu-Trp-His-Ade. AD + BD, AD + BD-PtoCYCD2;1 and AD-PtoCDKs + BD, were used as negative control. Day represents the number of days of growth on the corresponding medium. The triangle represents dilution in a 0.1-fold gradient (1,0.1 and 0.01).