Table 1 Fluorescence lifetime components of Ca2+-discharged obelin and obelin-v fluorescence.

From: Crystal structure of semi-synthetic obelin-v after calcium induced bioluminescence implies coelenteramine as the main reaction product

Ca2+-discharged protein

Spectral range/excitation wavelength

τi, ns

fi, %

λimax, nm

χ2

Obelin

394–582 nm/373 nm

1.52

12.4

482

0.99

4.65

87.6

512

Obelin-v

413–650 nm/407 nm

0.27

5.1

440

1.04

1.62

26.4

434

3.23

23.1

440

8.37

45.4

548

  1. τi—lifetime component calculated by global analysis of the decays within indicated spectral range; fi—spectral fraction of the component with τi in total emission spectrum; λimax—spectrum maximum, associated with τi, (DASi); χ2—statistical criterion characterizing the quality of analysis using indicated lifetime components and their amplitudes.
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