Figure 3 | Scientific Reports

Figure 3

From: dTMP imbalance through thymidylate 5′-phosphohydrolase activity induces apoptosis in triple-negative breast cancers

Figure 3The alternative text for this image may have been generated using AI.

Expressional changes of essential pyrimidine biosynthetic enzymes regulated by oncogene driven signaling. (A) RNAseq analysis of pyrimidine metabolic pathway (6 h/12 h/24 h). AKG: α-ketoglutarate, GLU: glutamate, GLN: glutamine, CarP: carbamoyl phosphate, OAA: oxaloacetate, ASP: aspartate, C-ASP: carbamoyl-aspartate, DHO: dihydroorotate, ORO: orotate, TD: thymidine, T: thymine, GLUD: glutamate dehydrogenase, GLUL: glutamate-ammonia ligase, GOT: glutamic-oxaloacetic transaminase, CAD: carbamoyl-phosphate synthetase, aspartate transcarbamylase, and dihydroorotase complex, DHODH: dihydroorotate dehydrogenase, UMPS: UMP synthetase, RRM1/2: ribonucleotide reductase catalytic subunit M1/2, RRM2B: ribonucleotide reductase regulatory TP53, NME: nucleoside diphosphate kinase, DUT: dUTPase, TYMS: thymidylate synthase, DTYMK: deoxythymidylate kinase, TK1: cytosolic thymidine kinase, TYMP: thymidine phosphorylase, SAMHD: SAM and HD domain containing dNTP triphosphohydrolase, T001: enzyme translated from CT. (B) Western blot analysis of MDA-MB-231 cells was carried out to investigate the expression changes of essential pyrimidine biosynthetic enzymes and the oncogene-driven signaling proteins. Original blots are presented in Supplementary Information (Figure Raw data).

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