Figure 4 | Scientific Reports

Figure 4

From: dTMP imbalance through thymidylate 5′-phosphohydrolase activity induces apoptosis in triple-negative breast cancers

Figure 4The alternative text for this image may have been generated using AI.

MDA-MB-231 maximal metabolic profiles with CT transfection. (A) Schematic indicating derivation of maximal metabolic rates, glycolytic reserve, and spare respiratory capacity from the experiment in (B,C). (B) ECAR assay for MDA-MB-231 transfected with CT. Cells were maintained in uniform media prior to measuring ECAR. ECAR was measured twice in the basal state, and then twice again following each metabolic inhibitor treatment (0.5 μM rotenone/0.5 μM antimycin A, 50 mM 2-DG) at 7 min intervals. (C) OCR assay for MDA-MB-231 transfected with CT. OCR was measured twice in the basal state, and then twice again following each metabolic inhibitor treatment (1.5 µM oligomycin, 0.5 μM FCCP, 0.5 μM rotenone/0.5 μM antimycin A) at 7 min intervals. ECAR and OCR values were normalized to a measurement of total cell numbers for each cell line in each assay. Data are their averages from one representative experiment, with error bars representing SD.

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