Figure 7 | Scientific Reports

Figure 7

From: dTMP imbalance through thymidylate 5′-phosphohydrolase activity induces apoptosis in triple-negative breast cancers

Figure 7The alternative text for this image may have been generated using AI.Figure 7The alternative text for this image may have been generated using AI.

Effects of CT on energy metabolism according to TNBC cell subtypes. (A) Quantification of total dNTP levels of three TNBC cell lines with different genetic backgrounds. Considering Lehmann classification, three representative cell lines of TNBC subtypes were determined as BT-549 (mesenchymal stem-like (M); basal B), MDA-MB-231 (mesenchymal stem-like (MSL); basal B) and MDA-MB-468 (basal-like1 (BL1); basal A) cells. (B) Basal ECAR and basal OCR analysis of three TNBC cell lines. (C) Maximal ECAR and basal OCR analysis of three TNBC cell lines. (D) Glycolytic reserve and spare respiratory capacity rate of three TNBC cell lines. Metabolic profiles were measured using an extracellular flux analyzer (Seahorse Bioscience). ECAR and OCR values were normalized to a measurement of total cell numbers for each cell line in each assay. Data are averages from one representative experiment, with error bars representing SD. (E) Metabolic dependency of three TNBC cells tested. Original blots are presented in Supplementary Information (Figure Raw data).

Back to article page