Figure 1

Spatial sectioning method and representative sectioned images. (a) Spatial sectioning procedure. (b) Preservation of spatial information pertaining to the sectioned specimens. Sectioned organs (top) and macro images of hematoxylin and eosin (H&E)-stained sections (bottom) from nude BALB/c mice with ES-2 ovarian cancer dissemination are shown. I Intestine, K kidney, L liver, P pancreas, S spleen. Scale bar: 2 mm. (c) Preserved metastatic niches composed of multiple organs/tissues. T disseminated cancer, U uterus, R retroperitoneal adipose tissue, PO periovarian adipose tissue, OT ovarian tube, L liver, P pancreas, PD pancreatic duct, MO major omentum, S stomach. Scale bar from left to right images: 500 µm, 100 µm, 500 µm, and 100 µm, respectively.