Table 6 Primers for real-time RT-PCR.
Symbol | Forward | Reverse | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
Sardh | GCTGGGTGTAGGTGGAGTTG | CTGGTTGGAGGCGATGAAG | 217 | 62 |
Dmgdh | ACAAGGGCAAGGTGATTGG | CTGGTTCTGGTAGGTTCTGTGAG | 193 | 58 |
Bhmt | TGTGGTAGGGCTCAAATCC | CCATTGTCGGTGTGAACTG | 288 | 60 |
Cbs | CGGTGGTGGATAGGTGGTTC | ACAGAGTCGGGCAGGATGAC | 181 | 60 |
Gnmt | CGCTAAAGAACATCGCAAGC | GTGGGTAGTAAGAGAGCCGAAAC | 267 | 60 |
Gamt | TGAAGAGACCTGGCACACTC | CATCTGAGGGAAGGCATAGTAG | 265 | 60 |
Pemt | TAGCAAGGTGGGAGCAGAG | GCTGGAGAGCACAAACACG | 227 | 58 |
Pcyt1a | TCAAGGAAGAGGAGGAAAGAGG | GGCGTGACCAGAGTGAAATAAG | 249 | 58 |
Mthfr | GAGTGTCTTTGAGGTCTTTGAGC | TGGGAGTTGATGGTGAGGA | 175 | 60 |
Smek2 | CTGCATATCAGAAGCAGCAG | ACTGATGGGTCCTTACCTTG | 142 | 62 |
Gusb | GCAGTTGTGTGGGTGAATGGG | GGGGTCAGTGTGTTGTTGATGG | 143 | 62 |
