Figure 2
From: LAMP3 transfer via extracellular particles induces apoptosis in Sjögren’s disease
Culture medium of LAMP3-overexpressing A253 cells induce apoptosis in naïve A253 cells. A253 cells were transfected with control empty plasmid, LAMP3-encoding plasmid or LAMP1-encoding plasmid. (A) Representative Western blots of transfected A253 cells (uncropped images are provided in Supplementary Fig. 3) and schematic methods of the following in vitro assays. (B) Naïve A253 cells were treated with culture medium of control or LAMP3-overexpressing A253 cells for 48 h (n = 3). (C) Naïve A253 cells were treated with culture medium of control, LAMP1-overexpressing, or LAMP3-overexpressing A253 cells or with post-ultracentrifugation supernatant or post-ultracentrifugation pellet from LAMP3-overexpressing A253 cells for 48 h (n = 4). Apoptotic cells in naïve cell culture were determined by flow cytometry using APC Annexin V/7-AAD. Graphs show difference in mean (± SD) number of Annexin V+/7-AAD+ cells in cell culture compared with control. **p < 0.01 (one-way ANOVA).
