Figure 1

RNase T1 Oligonucleotide Map of BNT162b2 Original DS. (A) IP RP-UHPLC-UV-MS/MS RNase T₁ oligonucleotide map of BNT162b2 Original DS, 14–254 min. “R” represents oligonucleotide RNase T₁ digestion products indexed from the 5′ to 3′ end. “*” denotes a sequence-repeat oligonucleotide, where the single peak assignment represents all identical oligonucleotides in the sequence. Each color distinguishes the number of nucleotides per digestion product: blue: 4, 10 and 16; green: 5 and 11; gold: 6 and 12; red: 7 and 13, purple: 8 and 14, black: 9 and 15, magenta: > 16. For graphical clarity, not all observed oligonucleotides are annotated on the chromatogram; a complete list is in Supplementary Data Table 1. (B) IP RP-UHPLC/UV/MS/MS RNase T₁ oligonucleotide map of BNT162b2 Original DS, 0–20 min. “R” represents oligonucleotide RNase T₁ digestion products indexed from the 5′ to 3′ end. “*” denotes a sequence-repeat oligonucleotide, where the single peak assignment represents all identical oligonucleotides in the sequence. Each color distinguishes the number of nucleotides per digestion product: red: 1; purple: 2; black: 3; blue: 4; green: 5. For graphical clarity, not all observed oligonucleotides are annotated on the chromatogram; a complete list is in Supplementary Data Table 1. (C) The 55.6% unique sequence coverage is illustrated as shaded by blue and green, based on 314 observed unique-sequence oligonucleotides. Blue nucleotides comprise unique-sequence RNase T₁ oligonucleotides; green nucleotides comprise unique-sequence missed-cleavage and fragment oligonucleotides. Green and white nucleotides also comprise repeat-sequence RNase T₁ oligonucleotides, based on 74 observed repeat-sequence oligonucleotides. “V” is N1-methyl pseudouridine.