Figure 2 | Scientific Reports

Figure 2

From: Short-term effects of occlusion therapy and optical correction on microvasculature in monocular amblyopia: a retrospective case–control study

Figure 2The alternative text for this image may have been generated using AI.

Representative images of choroidal microvasculature measured using OCTA. (a–c) Using the IMAGEnet program, CT was manually measured from the start of the choriocapillary layer just beneath the retinal pigment epithelial-Bruch's membrane complex to the choroid-sclera boundary, relative to the macular surface. The measurement was repeated at a location 300 µm to the nasal and temporal side of the macula, and the average of all three measurements was used. (d–i) CVI was manually measured using ImageJ. A 1500 µm horizontal length of the choroid was used as the reference, covering 750 µm on both the nasal and temporal sides of the macula (which was demarcated by a yellow border). The image was converted to a binary format using Niblack's method in ImageJ (g–i). The ratio of black pixels (vascular area) to the sum of black and white pixels (total area) inside the yellow area was calculated (a, d, g: patch occlusion group; b, e, h: optical correction group; c, f, i: control group). The images were created using the built-in software program IMAGEnet6 (a–f) (version 1.25.16650; Topcon, URL: https://topconhealthcare.jp/products/imagenet-6/) in the DRI OCT Triton Plus instrument (Topcon Co., Tokyo, Japan), and processed using the ImageJ program (g–i) (version 1.52a; National Institutes of Health, Bethesda, MD, URL: https://imagej.nih.gov/ij/download.html). OCTA, optical coherence tomography angiography; CT, choroidal thickness; CVI, choroidal vascularity index.

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