Figure 6

Effects of FC on the expression of Filaggrin in vivo and in vitro models. (A,B) Total proteins were prepared and immunoblotted for Filaggrin by using specific antibodies. β-Actin was used as internal control. The protein levels were quantified by band density in proportion to the expression of total form. The original result images of all western blots are attached in the Supplementary Figures. (C) Representative histologic section findings of skin dorsal tissue immunostained with Filaggrin antibody using immunohistochemical staining (scale bar = 10 μm). The data shown represent mean ± SD of three independent experiments. ###p < 0.001 vs the control group; ***p < 0.001 vs TNF-α/IFN-γ-stimulated group.