Figure 4

TUNEL staining after spinal cord injury and immunofluorescence staining for NeuN and GFAP in the ventral horn of the injury epicenter. (A) Representative photo of NeuN (in red), GFAP (in green) and DAPI (in blue) staining at 4 weeks after SCI in different groups. (B) Quantitative analysis of neuronal cells. (C) Quantitative analysis of GFAP relative intensity. (D) Representative TUNEL-positive cells (in green) in spinal cord sections at 4 weeks after SCI. Nuclei were stained with DAPI (in blue). (E) Quantitative analysis of the TUNEL/DAPI (GFAP glial fibrillary acidic protein, DAPI 4',6-diamidine-2'-phenylindole dihydrochloride, SCI spinal cord injury; *P < 0.05, **P < 0.01, ***P < 0.001).