Figure 3
From: Modeling mutation-specific arrhythmogenic phenotypes in isogenic human iPSC-derived cardiac tissues
Modeling L/SQTS in 2D on MEA. (a) Preparation for Multi-electrode device (MED) Analysis in 2D culture. (b) Representative microscopic bright field appearance of 2D cardiomyocytes on MEA electrode (64ch). (c) Representative field potential waveforms of 2D cardiomyocytes on MEA electrode (64ch). (d) Representative field potential waveform of 2D cardiomyocyte from gene edited hiPSCs (Parent vs SQT vs LQT). (e) FPDcF quantification Parent/SQT/LQT (box plot, statistical analysis). Parent (n = 7), SQT22 (n = 12), LQT26 (n = 9), Dunnett’s test *P < 0.05, **P < 0.01 and ***P < 0.001. ”n” is the number of replicate probes used for each clone. Scale bars: 150 μm in (c).
